bio rad trans blot turbo electrotransfer system Search Results


transit cos transfection reagent  (Mirus Bio)
96
Mirus Bio transit cos transfection reagent
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Transit Cos Transfection Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds polyacrylamide gel electrophoresis  (Bio-Rad)
95
Bio-Rad sds polyacrylamide gel electrophoresis
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Sds Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean tgx precast gels  (Bio-Rad)
99
Bio-Rad mini protean tgx precast gels
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Mini Protean Tgx Precast Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean tgx precast gels - by Bioz Stars, 2026-06
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nitrocellulose membrane  (Bio-Rad)
99
Bio-Rad nitrocellulose membrane
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Nitrocellulose Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membrane - by Bioz Stars, 2026-06
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electrotransfer device  (Bio-Rad)
99
Bio-Rad electrotransfer device
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Electrotransfer Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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electrotransfer device - by Bioz Stars, 2026-06
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blotting grade nonfat dry milk  (Bio-Rad)
96
Bio-Rad blotting grade nonfat dry milk
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Blotting Grade Nonfat Dry Milk, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinylidene difluoride membranes  (Bio-Rad)
96
Bio-Rad polyvinylidene difluoride membranes
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Polyvinylidene Difluoride Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinylidene difluoride pvdf membranes  (Bio-Rad)
96
Bio-Rad polyvinylidene difluoride pvdf membranes
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Polyvinylidene Difluoride Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nytran n filter  (Bio-Rad)
96
Bio-Rad nytran n filter
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Nytran N Filter, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membranes  (Bio-Rad)
96
Bio-Rad nitrocellulose membranes
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Nitrocellulose Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membranes - by Bioz Stars, 2026-06
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glycine buffer  (Bio-Rad)
97
Bio-Rad glycine buffer
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Glycine Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glycine buffer - by Bioz Stars, 2026-06
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tris  (Bio-Rad)
98
Bio-Rad tris
Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h <t>post-transfection,</t> released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.
Tris, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris - by Bioz Stars, 2026-06
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Image Search Results


Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h post-transfection, released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.

Journal: The Journal of general virology

Article Title: Two palmitylated cysteine residues of the severe acute respiratory syndrome coronavirus spike (S) protein are critical for S incorporation into virus-like particles, but not for M-S co-localization.

doi: 10.1099/vir.0.038091-0

Figure Lengend Snippet: Fig. 2. WT and mutant S incorporation into SCoV VLPs. (a) Plasmids encoding S, M, N or E proteins were cotransfected into 293T cells. At 72 h post-transfection, released VLPs and cellular extracts were subjected to SDS-PAGE, electrotransfer of the separated proteins onto a transfer membrane and Western blot. S(”) VLPs were generated with empty pCAGGS vectors instead of pCAGGS-SCoV S. (b) ‘Only S’ indicates that plasmids encoding S and empty pCAGGS vectors were transfected into 293T cells.

Article Snippet: Transient S expression was performed using TransIT COS Transfection Reagent (Mirus Bio), according to the manufacturer’s instructions.

Techniques: Mutagenesis, Transfection, SDS Page, Electrotransfer, Membrane, Western Blot, Generated